entre
ou
cadastre-se

Padrões

AC-1 AC-2 AC-3 AC-4 AC-5 AC-6 AC-7 AC-8 AC-9 AC-10 AC-11 AC-12 AC-13 AC-14 AC-15 AC-16 AC-17 AC-18 AC-19 AC-20 AC-21 AC-22 AC-23 AC-24 AC-25 AC-26 AC-27 AC-28
AC-29
BAC-1 BAC-2 BAC-3 BAC-4 BAC-5

AC-29 - TOPOI-like

Descrição

The Topo I-like pattern consists of staining in five subcellular regions:

Nuclear staining (AC-4 type) – Prominent fine speckled pattern in interphase cells.

Mitotic chromatin staining – Strong fine speckled staining in condensed chromatin, which may appear homogeneous depending on serum dilution.

Nucleolar organizing region (NOR) staining – Strong staining on condensed chromosomes in mitotic cells, though it may be obscured by bright chromosomal staining due to focal plane differences.

Cytoplasmic staining – Weak staining in interphase and mitotic cells, forming a delicate network radiating from the perinuclear area. Higher serum dilutions may enhance cytoplasmic visibility.

Nucleolar staining – Variable punctate or perinucleolar staining in interphase cells, though not universally present in this pattern.

This five-element pattern appears in most commercial HEp-2 slides, though variations exist by brand. Detecting all elements can be challenging, especially with a single serum dilution (e.g., strong mitotic chromatin staining masking NOR) or in semi-automated systems using a single focal plane (e.g., NOR or cytoplasmic staining out of focus).

Associação Antigênica

DNA topoisomerase I

Doenças Associadas

SSc, in particular with diffuse cutaneous SSc

Relevâncias Clínicas - Primeiro nível

The AC-29 pattern is highly specific for SSc, in particular with diffuse cutaneous SSc and more aggressive forms of SSc
Andrade et al., 2018
Dellavance et al., 2009
Johnson et al., 2012
If SSc is clinically suspected, it is recommended to perform a follow-up test for anti-Topoisomerase I (formerly Scl-70) antibodies; the anti-Topoisomerase I antibodies are included in the classification criteria for SSc and the antigen is included in routine ENA profiles
van den Hoogen et al, 2013
If SSc is clinically suspected, it is recommended to perform a follow-up test for anti-Topoisomerase I (formerly Scl-70) antibodies; the anti-Topoisomerase I antibodies are included in the classification criteria for SSc and the antigen is included in routine ENA profiles
Johnson et al., 2012
Basu & Reveille, 2005

Referências

Andrade LEC, Klotz W, Herold M, et al. International consensus on antinuclear antibody patterns: definition of the ac-29 pattern associated with antibodies to DNA topoisomerase I. Clin Chem Lab Med 2018;56:1783–8. Dellavance A, Gallindo C, Soares MG, et al. Redefining the Scl-70 indirect immunofluorescence pattern: autoantibodies to DNA topoisomerase I yield a specific compound immunofluorescence pattern. Rheumatology 2009;48:632–7. Johnson SR, Fransen J, Khanna D, et al. Validation of potential classification criteria for systemic sclerosis. Arthritis Care Res 2012;64:358–67. van den Hoogen F, Khanna D, Fransen J, et al. 2013 classification criteria for systemic sclerosis: an American College of rheumatology/European League against rheumatism collaborative initiative. Ann Rheum Dis 2013;72:1747–55. Johnson SR, Fransen J, Khanna D, et al. Validation of potential classification criteria for systemic sclerosis. Arthritis Care Res 2012;64:358–67. Basu D, Reveille JD. Anti-scl-70. Autoimmunity 2005;38:65–72.