PORTUGUÊS / ENGLISH

AC-10 - Punctate Nucleolar

Descrição
Densely distributed but distinct grains seen in the nucleoli of interphase cells. In metaphase cells, up to 5 bright pairs of the nucleolar organizer regions (NOR) can be seen within the chromatin body. The cytoplasm of mitotic cells may be slightly positive. e.g. anti-NOR-90, anti-RNA polymerase I.
Associação Antigênica
RNA polymerase I, hUBF/NOR-90
Doença Associada
SSc, SjS
Relevância Clínica (Primeiro Nível)
The AC-10 pattern can be seen in various conditions, including SSc, Raynaud’s phenomenon, SjS, and cancer.
Reimer, Rose, Scheer et al., 1987Kuwana, Kaburaki, Mimori et al., 1993Fritzler, von Munlen, Toffoli et al., 1995Fujii, Mimori & Akizuki, 1995 Imai, Ochs, Kiyosawa et al., 1996
If the AC-10 pattern is observed in the serum of patients with conditions mentioned above, follow-up testing for anti-NOR90 (hUBF) antibodies is to be considered; the antigen is included in disease specific immunoassays (i.e. SSc profile).
Fritzler, von Munlen, Toffoli et al., 1995Fujii, Mimori & Akizuki, 1995
While AC-10 is associated with anti-RNApol I antibodies, these antibodies almost always coexist with anti-RNApol III antibodies which reveal the AC-5 pattern; therefore, if SSc is clinically suspected, it is recommended to perform a follow-up test for anti-RNApol III antibodies (See also AC-5); specific immunoassays for anti-RNA pol I antibodies are currently not commercially available.
Reimer, Rose, Scheer et al., 1987 Kuwana, Kaburaki, Mimori et al., 1993Yamasaki, Honkanen-Scott, Hernandez et al., 2006
Referências
Fritzler MJ, von Muhlen CA, Toffoli SM, et al. Autoantibodies to the nucleolar organizer antigen NOR-90 in children with systemic rheumatic diseases. J Rheumatol 1995;22:521–4.

Fujii T, Mimori T, Akizuki M. Detection of autoantibodies to nucleolar transcription factor nor 90/hUBF in sera of patients with rheumatic diseases, by recombinant autoantigen-based assays. Arthritis Rheum 1996;39:1313–8.

Imai H, Ochs RL, Kiyosawa K, et al. Nucleolar antigens and autoantibodies in hepatocellular carcinoma and other malignancies. Am J Pathol 1992;140:859–70.

Kuwana M, Kaburaki J, Mimori T, et al. Autoantibody reactive with three classes of RNA polymerases in sera from patients with systemic sclerosis. J Clin Invest 1993;91:1399–404.

Reimer G, Rose KM, Scheer U, et al. Autoantibody to RNA polymerase I in scleroderma sera. J Clin Invest 1987;79:65–72.

Yamasaki Y, Honkanen-Scott M, Hernandez L, et al. Nucleolar staining cannot be used as a screening test for the scleroderma marker anti-RNA polymerase I/III
antibodies. Arthritis Rheum 2006;54:3051–6.